Transcriptomic analysis of nonylphenol effect on Saccharomyces cerevisiae

Nonylphenol (NP) is a bioaccumulative environmental estrogen that widely used as nonionic surfactant. We have previously examined short-term effects of NP on yeast cells using microarray technology. In the present study, we investigated adaptive response Saccharomyces cerevisiae BY4742 to exposure by analyzing genome-wide transcriptional profiles RNA-sequencing. 2 mg/L concentration for 40 days exposure. Gene expression analysis showed total 948 genes were differentially expressed. Of these, 834 downregulated, while 114 significantly upregulated. GO enrichment revealed 369 terms affected Further many expressed associated with oxidative phosphorylation, iron and copper acquisition, autophagy, pleiotropic drug resistance cell cycle progression related processes such DNA mismatch repair, chromosome segregation, spindle checkpoint activity, kinetochore organization. Overall, these results provide considerable information comprehensive understanding at gene level.